LOCUS       POMT1-pGEc2             7467 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6945..7074)
                     /note="3'-ITR"
     misc_feature    complement(6643..6857)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    6095..6636
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    5274..5296
                     /note="attB2"
     misc_feature    6074..6079
                     /note="BstB1 site"
     misc_feature    5297..5992
                     /note="Super GFP"
     misc_feature    5993..6043
                     /note="AviTag"
     misc_feature    6044..6067
                     /note="His Tag"
     PCR_frame       3011..5273
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..5251
                     /note="OPTIMIZED POMT1 coding region"
     misc_feature    5252..5273
                     /note="TEV"
     vector          join(5282..7467,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..5281
                     /source="POMT1 pDONR"
                     /type="Entry Clone"
     source          1..7467
                     /dnas_title="POMT1-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGTGGGGCT TTCTGAAAAG ACCTGTGGTG GTCACCGCCG ACATCAATCT
     3061 GTCTCTGGTG GCTCTGACAG GCATGGGCCT GCTGTCTAGA CTGTGGCGGC TGACATATCC
     3121 CAGAGCCGTG GTGTTCGATG AGGTGTACTA CGGCCAGTAC ATCAGCTTCT ATATGAAGCA
     3181 GATCTTCTTC CTGGACGACT CCGGCCCTCC TTTCGGACAT ATGGTGCTGG CACTGGGCGG
     3241 CTACCTGGGC GGATTTGATG GCAACTTCCT GTGGAACCGG ATCGGAGCCG AGTACAGCTC
     3301 TAATGTGCCT GTGTGGTCCC TGAGACTGCT GCCTGCTCTG GCTGGTGCTC TGTCTGTGCC
     3361 TATGGCCTAC CAGATCGTGC TGGAACTGCA CTTTAGCCAC TGTGCCGCTA TGGGAGCTGC
     3421 CCTGCTGATG CTGATCGAGA ATGCCCTGAT CACCCAGTCC CGGCTCATGC TGCTGGAAAG
     3481 CGTGCTGATC TTTTTCAACC TGCTGGCCGT GCTGAGCTAC CTGAAGTTCT TCAACTGCCA
     3541 GAAGCACAGC CCCTTCAGCC TGAGCTGGTG GTTTTGGCTG ACACTGACTG GCGTGGCCTG
     3601 TTCTTGTGCC GTGGGCATCA AGTACATGGG CGTGTTCACA TACGTGCTGG TCCTGGGAGT
     3661 GGCTGCCGTG CATGCTTGGC ATCTGCTGGG AGATCAGACC CTGTCCAATG TGGGAGCCGA
     3721 TGTGCAGTGT TGTATGAGGC CTGCCTGTAT GGGCCAGATG AGAATGAGTC AGGGCGTGTG
     3781 CGTGTTCTGC CATCTGCTCG CTAGAGCAGT CGCCCTGCTC GTGATTCCAG TGGTGCTGTA
     3841 CCTGCTGTTC TTCTACGTGC ACCTGATCCT GGTGTTCAGA AGCGGCCCTC ACGACCAGAT
     3901 CATGTCTAGC GCCTTTCAGG CCTCTCTGGA AGGCGGACTG GCCAGAATTA CACAGGGACA
     3961 GCCTCTCGAG GTGGCCTTCG GAAGCCAGGT CACACTGAGA AACGTGTTCG GCAAGCCTGT
     4021 GCCTTGTTGG CTGCACTCTC ACCAGGACAC CTACCCCATG ATCTACGAGA ATGGCAGAGG
     4081 CAGCAGCCAC CAGCAGCAGG TCACCTGTTA CCCCTTCAAG GACGTGAACA ACTGGTGGAT
     4141 CGTGAAGGAC CCTCGGAGGC ATCAGCTCGT GGTGTCATCT CCTCCAAGAC CAGTGCGGCA
     4201 TGGCGATATG GTCCAGCTGG TGCACGGAAT GACCACAAGA AGCCTGAATA CCCACGACGT
     4261 GGCCGCTCCT CTGTCTCCAC ATAGCCAGGA AGTGTCCTGC TACATCGACT ACAACATCAG
     4321 CATGCCCGCC CAGAACCTCT GGCGCCTGGA AATCGTGAAC AGAGGCTCTG ATACCGACGT
     4381 GTGGAAAACC ATCCTGAGCG AAGTCCGCTT CGTGCATGTG AATACCAGCG CTGTGCTGAA
     4441 GCTGAGCGGA GCCCATCTGC CTGATTGGGG CTACAGACAG CTGGAAATTG TGGGCGAGAA
     4501 GCTGTCAAGA GGCTACCACG GAAGCACTGT GTGGAATGTG GAAGAACACA GATACGGCGC
     4561 CTCTCAGGAA CAGCGCGAGA GAGAAAGAGA GCTGCATTCT CCAGCACAGG TGGACGTGTC
     4621 CCGGAATCTG AGCTTCATGG CCAGATTCTC TGAGCTGCAG TGGCGGATGC TGGCCCTGAG
     4681 ATCTGATGAC AGCGAGCACA AGTACTCCAG CTCCCCCCTG GAATGGGTCA CCCTGGATAC
     4741 CAATATCGCC TACTGGCTGC ATCCTCGGAC CAGCGCCCAG ATTCACCTCC TGGGAAATAT
     4801 CGTGATCTGG GTGTCCGGAT CTCTGGCCCT CGCTATCTAT GCTCTGCTGA GCCTGTGGTA
     4861 TCTGCTGCGG CGGAGAAGAA ATGTGCACGA CCTGCCACAG GATGCCTGGC TGAGATGGGT
     4921 CCTGGCAGGC GCTCTGTGTG CTGGGGGATG GGCTGTGAAC TACCTGCCAT TTTTCCTGAT
     4981 GGAAAAAACC CTGTTTCTGT ACCACTATCT GCCAGCCCTG ACCTTTCAGA TCCTGCTCCT
     5041 GCCAGTGGTC CTGCAGCACA TCAGCGATCA CCTGTGTAGA AGCCAGCTGC AGCGGTCCAT
     5101 CTTTTCTGCA CTGGTGGTGG CCTGGTACTC CTCCGCCTGT CATGTGTCTA ATACCCTGAG
     5161 GCCTCTGACC TACGGCGACA AGAGCCTGTC TCCTCACGAA CTGAAAGCCC TGAGGTGGAA
     5221 GGATAGCTGG GACATCCTGA TCCGCAAGCA Cgaaaacttg tactttcaag gctCCAGCTT
     5281 TCTTGTACAA AGTGGTATGA GCAAGGGCGA GGAACTGTTC ACCGGCGTGG TGCCCATCCT
     5341 GGTGGAACTG GATGGCGACG TGAACGGCCA CAAGTTCTCT GTGCGGGGAG AGGGCGAAGG
     5401 CGACGCCACA AATGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT
     5461 GCCTTGGCCT ACCCTCGTGA CCACACTGAC CTACGGCGTG CAGTGCTTCA GCAGATACCC
     5521 CGACCATATG AAGCGGCACG ACTTCTTCAA GAGCGCCATG CCCGAGGGCT ACGTGCAGGA
     5581 ACGGACCATC AGCTTCAAGG ACGACGGCAC CTACAAGACC AGAGCCGAAG TGAAGTTCGA
     5641 GGGCGACACC CTCGTGAACC GGATCGAGCT GAAGGGCATC GATTTCAAAG AGGACGGCAA
     5701 CATCCTGGGC CACAAGCTGG AGTACAACTT CAACAGCCAC AACGTGTACA TCACCGCCGA
     5761 CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGG CACAACGTGG AAGATGGCAG
     5821 CGTGCAGCTG GCCGACCACT ACCAGCAGAA CACCCCCATC GGAGATGGCC CCGTGCTGCT
     5881 GCCCGACAAC CACTACCTGA GCACCCAGAG CGTGCTGAGC AAGGACCCCA ACGAGAAGCG
     5941 GGACCACATG GTGCTGCTGG AATTCGTGAC CGCCGCTGGC ATCACCCACG GCATGAGCGG
     6001 CCTGAACGAC ATCTTCGAGG CCCAGAAAAT CGAGTGGCAC GAGCACCACC ATCATCACCA
     6061 TCACCACTGA TGATTCGAAA AATAATAAGG ATCCAATCAA CCTCTGGATT ACAAAATTTG
     6121 TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC
     6181 TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA
     6241 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT
     6301 GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA
     6361 GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC
     6421 CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT
     6481 GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG
     6541 CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG
     6601 CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGAGAT CTGCCTCGAC TGTGCCTTCT
     6661 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC
     6721 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT
     6781 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT
     6841 AGCAGGCATG CTGGGGACTC GAGTTAAGGG CGAATTGCCG ATTAGGATCT TCCTAGAGCA
     6901 TGGCTACGTA GATAAGTAGC ATGGCGGGTT AATCATTAAC TACAAGGAAC CCCTAGTGAT
     6961 GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT
     7021 CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCCTTAA
     7081 TTAAGAGCTC ATGGCGCGCC TAGGCCTTGA CGGCCTTCCG CCAATTCGCC CTATAGTGAG
     7141 TCGTATTACG TCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
     7201 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
     7261 AGAGGCCCGC ACCGAAACGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGAGC
     7321 GCCCTGTAGC GGCCACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
     7381 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
     7441 AACAAAATAT TAACGCTTAC AATTTAG
//

